Part:BBa_K1124000
amilGFP (+LVA), yellow reporter protein with degradation tag
Usage and Biology
The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction).
BBa_K1124000 is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction).
amilGFP
amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family.
Excitation maximum : 503 nm Emission maximum : 512 nm Quantum yield : 0.67 Molar Extinction : 75200
LVA tag
LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
References
Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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