Regulatory

Part:BBa_K1086000:Design

Designed by: Ítalo Faria do Valle   Group: iGEM13_UFMG_Brazil   (2013-09-11)
Revision as of 13:51, 11 September 2013 by Registry (Talk | contribs)

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Promoter TorCAD (sensitive to TMAO)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to have 125 nucleotides for synthesis by IDT-DNA we had to add 5 randomly nucleotides in the 5' end of the sequence right above EcoRI cutting site.


Source

The source of this part is the genome of Escherichia coli (str. K-12 substr. MG1655), NCBI GI: 48994873. (Bordi et al. Mol. Micro. 2003)

References