Composite

Part:BBa_K1074007

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-11)
Revision as of 09:31, 11 September 2013 by Registry (Talk | contribs)

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Pctc+RBS+amilCP

Promoter ctc is a sigma factor B-dependent promoter found in B. subtilis. In B. subtilis endogenous sigma factor B is activated under mild stress.amilCP, blue chromoprotein,naturally exhibits strong color when expressed.We assemble this gene circuit to get a visual signal when B.subtilis grow to the post-log phase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None