Coding

Part:BBa_K1054001

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-10)
Revision as of 02:01, 11 September 2013 by Registry (Talk | contribs)

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protein E of Phage phiX174 under pBAD

Prontein E is a membrane protein that inhibits MraY, an enzyme required for murein synthesis, and promotes cell lysis in a manner analogous to cell wall synthesis inhibitors like penicillin. However, it will keep the periplasmic space of Gram-negative strain intact. In this part, protein E will be expressed when L-arabinose is available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
Parameters
n/aprotein E of Phage phiX174 under pBAD