Plasmid

Part:BBa_K1223008

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-09)
Revision as of 07:45, 9 September 2013 by Registry (Talk | contribs)

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pUC-57-P.A.S.E.2

P.A.S.E2 cassette cloned into pUC-57 (ampicillin resistance).

the cassette is cloned between BamHI and XbaI restriction sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 1238
    Illegal PstI site found at 1637
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 1238
    Illegal PstI site found at 1637
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BglII site found at 1451
    Illegal BamHI site found at 1619
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 1238
    Illegal PstI site found at 1637
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 1238
    Illegal PstI site found at 1637
    Illegal NgoMIV site found at 787
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2974
    Illegal SapI site found at 727
    Illegal SapI site found at 937
    Illegal SapI site found at 1891


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