Composite

Part:BBa_K1140002:Design

Designed by: Janssel Reyes del Castillo   Group: iGEM13_UANL_Mty-Mexico   (2013-09-08)
Revision as of 19:22, 8 September 2013 by Registry (Talk | contribs)

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pLac + 32 oC RNA thermometer + GFP(LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.


Source

Obtained synthetically. Promoter pLac. Based on a fusion of a RBS and a Riboswitch. GFP (LVA). T7 double terminator(Further information coming soon).

References