Coding
ATF1

Part:BBa_J45006:Design

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)
Revision as of 15:31, 26 June 2006 by Drephd (Talk | contribs) (→‎Design Notes)


alcohol acetyltransferase I (ATF1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

  • Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
  • forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA G -3'
  • reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG -3'
  • forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
  • reverse mutagenesis primer: 5' CCCTTACTATTTTTGAACTCTTCTAATATTTGCTTC 3'

Source

Source: S. cerevisiae, chromosome XV

Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]

References

<biblio>

    • Horton03 pmid=12937998

</biblio>