Composite

Part:BBa_K1123008

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 14:18, 8 September 2013 by Registry (Talk | contribs)

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P(TK) Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Poly(Threonine-Lysine) (P(TK)) Protein sequence. The FNR promotor sequence enables us to bring the P(TK) protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The P(TK) protein is a protein containing 216 repeats of the Threonine-Lysine amino acid combination designed by us ourselves. It is being expressed with the sole purpose of using it to provide CEST contrast in an MRI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 641
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None