Composite

Part:BBa_K1123004

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 13:33, 8 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


1G70 Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the 1G70 Protein sequence. The FNR promotor sequence enables us to bring the 1G70 protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The 1G70 protein is part of a HIV protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 269
    Illegal NgoMIV site found at 353
    Illegal NgoMIV site found at 437
    Illegal NgoMIV site found at 521
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None