DNA

Part:BBa_K1123011:Design

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 12:16, 8 September 2013 by ArdjanvanderLinden (Talk | contribs) (→‎Design Notes)

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Spacer Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No considerations were made other than ensuring that no hair pins or dimers could be formed with the designed spacer. We also ensured no illegal restriction sites occurred. We did however add a stop codon and a XhoI restriction site to the very start of this sequence. The stop codon would allow us to stop protein expression wand the restriction site would allow us to restrict the protein sequence from the larger construct for transferal into a different vector. The Stop codon are the first 3 base pairs and the restriction site is formed by the next 6 base pairs.

Source

This part was designed by ourselves. It was constructed by using a randomising a DNA sequence.

References