DNA

Part:BBa_K1123012:Design

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)
Revision as of 12:12, 8 September 2013 by ArdjanvanderLinden (Talk | contribs) (→‎Design Notes)

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His-Tag and Thrombine Cleavage Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was left mainly unchanged as it was lifted directly from the pET28-a vector. However we did add a NheI restriction site to the end which we would be able to use to restrict the protein which we would be placing behind the His-Tag. These are the last 6 base pairs at the end of the sequence.

Source

The DNA sequence for this part was lifted directly from the pET28-a vector. This will allow us to use this part without further adaptations.

References