Regulatory

Part:BBa_K1114033:Design

Designed by: Traci Haddock   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 17:57, 7 September 2013 by THaddock (Talk | contribs) (→‎References)


MoClo formatted part of BBa_R0079 with FB fusion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


<a href="http://2013.igem.org/Team:Purdue">Purdue Biomaker's iGEM Team</a> ===Design Notes=== In a MoClo backbone, BBa_DVL0_FB. Purdue Biomaker's iGEM Team


MoClo formatted part of BBa_R0079 with FB fusion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_R0079 with FB fusion sites. See the BBa_R0079 page for full information on the part.

This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

Source

iGEM Kit

References