Regulatory

Part:BBa_K1114005:Design

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 17:19, 7 September 2013 by Jawtry (Talk | contribs) (→‎Design Notes)


The MoClo format of BBa_J23104 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_J23104 with EB fusion sites. See the BBa_J23104 page for full information on the part.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.

Source

iGEM Distribution Kit

References