Project

Part:BBa_K1223001

Designed by: Orr Schlesinger   Group: iGEM13_BGU_Israel   (2013-09-07)
Revision as of 10:25, 7 September 2013 by Registry (Talk | contribs)

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P.A.S.E. 1 cassette

A DNA cassette that undergo homologous recombination into E.coli genome that contains homologous regions at each end, Kanamicin resistance gene (KanR) and death operon containing Lambda holin and lysozime genes and controlled by a Lambda cI regulated promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//biosafety/kill_switch
//cds/enzyme/lysis
//cds/transcriptionalregulator/repressor
Parameters
negative_regulatorscI