Plasmid_Backbone

Part:BBa_K1138001:Design

Designed by: Rachit Jain   Group: iGEM13_UGA-Georgia   (2013-09-06)
Revision as of 16:48, 6 September 2013 by Rachit (Talk | contribs) (→‎Design Notes)

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pAW50-linker-mcherry(-RBS) (ugaiGEM11)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
    Illegal XbaI site found at 220
    Illegal SpeI site found at 969
    Illegal PstI site found at 609
    Illegal PstI site found at 976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
    Illegal SpeI site found at 969
    Illegal PstI site found at 609
    Illegal PstI site found at 976
    Illegal NotI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal EcoRI site found at 205
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 205
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal SpeI site found at 969
    Illegal PstI site found at 609
    Illegal PstI site found at 976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 205
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 199
    Illegal XbaI site found at 220
    Illegal SpeI site found at 969
    Illegal PstI site found at 609
    Illegal PstI site found at 976
    Illegal AgeI site found at 987
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3145
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 1857
    Illegal SapI site found at 4809


Design Notes

Primers were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker.

Source

mcherry was obtained from Discosoma sp.

References