Regulatory

Part:BBa_K1155000:Design

Designed by: Sheng, Abdou Mouthare, Sylvie Lautru   Group: iGEM13_Paris_Saclay   (2013-09-05)
Revision as of 14:13, 5 September 2013 by SylvieL (Talk | contribs) (→‎Design Notes)


Pndh*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter region corresponds to a modified ndh promoter region of Escherichia coli. The second FNR binding site (FNRII) was modified to obtain a perfect consensus motif, improving the strength of the repression (Meng et al., 1997).

Reference Meng W., Green J. and Guest J. R. (1997). FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites, Microbiology, 143, 1521-1532.

Source

Escherichia coli K12 genome sequence

References