Coding

Part:BBa_K1031911

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-01)
Revision as of 18:40, 1 September 2013 by Registry (Talk | contribs)

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XylS-Terminator

XylS senses benzoate and its substitutes, it is the transcriptional activation factor that activates the expression of meta-operon for benzoate degradation on TOL plasmid pWW0 in Pseudomonas putida. This part contains XylS coding sequence with terminator. To complete the sensor, please add promoter before XylS coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote
Parameters
biologyPseudomonas putida
proteinXylS