Coding

Part:BBa_K1123020

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-01)
Revision as of 18:11, 1 September 2013 by Registry (Talk | contribs)

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Poly(Threonine-Lysine) Protein

This part contains the DNA sequence of a protein of our own design. We first repeated the Threonine-Lysine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added. The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI. The DNA sequence can be placed behind any compatible promoter and the protein will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None