Part:BBa_K1065204

Efe+Bba_B0015 in BBa_K823024 (pXyl)

2-oxoglutarate oxygenase/decarboxylase is an Ethylene Forming Enzyme (EFE) that catalyze Ethylene biosynthesis from 2-oxoglutarate. This part is in the pXyl vector that has been sent to the Igem UNITN 2013 team from the LMU Munich 2012 team. This part was cloned by the iGEM Trento 2013 team for the creation of an aerobically engineered pathway for the control of fruit ripening. Further information about this part and its characterization can be found in the iGEM Trento 2013 wiki.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 5996
  Illegal suffix found in sequence at 1224
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5996
  Illegal SpeI site found at 1225
  Illegal PstI site found at 1239
  Illegal NotI site found at 1232
  Illegal NotI site found at 6002
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5996
  Illegal BglII site found at 319
  Illegal BamHI site found at 5980
  Illegal XhoI site found at 2234
  Illegal XhoI site found at 3152
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 5996
  Illegal suffix found in sequence at 1225
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 5996
  Illegal XbaI site found at 6011
  Illegal SpeI site found at 1225
  Illegal PstI site found at 1239
  Illegal NgoMIV site found at 17
  Illegal NgoMIV site found at 3116
  Illegal NgoMIV site found at 4255
  Illegal AgeI site found at 1070
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4079
  Illegal SapI.rc site found at 1828