Translational_Unit
BMST1

Part:BBa_K1065100:Design

Designed by: Caterina Marchioretti Michele Pedrotti   Group: iGEM13_UNITN-Trento   (2013-06-25)
Revision as of 14:02, 31 August 2013 by Cate (Talk | contribs) (→‎Design Notes)


BSMT1 enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 223


Design Notes

This part was built by starting from the extraction of BBa_J45119 from the IGEM registry 2012 Kit Distribution, then it was subcloned in linearized pSB1C3 by a reaction of digestion (EcoRI and PstI) followed by a reaction of ligation.

Source

b

References