Coding

Part:BBa_K1123014:Design

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)
Revision as of 12:08, 31 August 2013 by ArdjanvanderLinden (Talk | contribs) (→‎Design Notes)

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DNA Coding Sequence for 1ETF Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein sequence was codon optimized for expression in E.coli bacteria. We then repeated the sequence a number of times to amplify the protein. These repeats were held together using small linkage sequences of Glycine and Serine amino acids. In total the 1ETF protein sequence was repeated 5 times.

Source

The amino acid sequence for this parts was retrieved from PDB.org after a database query was performed looking for proteins with high Arginine and Lysine contents.

References