Coding

Part:BBa_K1123013:Design

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-08-31)
Revision as of 11:55, 31 August 2013 by Registry (Talk | contribs)

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Protamine-1-Optimized Protein Production Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did not adapt any part of the found amino acid sequence although we did optimize the codon usage for E.coli bacteria.


Source

The source for the amino acid sequence was found on www.UniProt.org. Using this amino acid sequence we were able to create an E.coli optimised version of this protein.

References