Generator
Part:BBa_J23005
Designed by: John Anderson Group: iGEM06_Berkeley (2006-06-23)
[FRT-CmR-FRT]
Biobricked cassette containing the chloramphenicol resistance gene flanked by FRT sites. Useful for constructing markerless genome-integration cassettes. The cassette is derived from pKD3 described by Datsenko&Wanner and can be excised by expression of flp recombinase in trans with helper plasmids such as pCP20.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 35
Illegal XbaI site found at 965 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 35
Illegal XbaI site found at 965 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 35
Illegal XbaI site found at 965 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |