Coding
Part:BBa_K1218014:Design
Designed by: Sophia Liang Group: iGEM13_Stanford-Brown (2013-08-29)
dCas9-ω Activator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1642
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3921
Illegal BamHI site found at 4755 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutagenesis to remove internal EcoRI site
Source
CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.