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Composite
luxR-ndh

Part:BBa_K1036000:Design

Designed by: Shengquan Zeng, Zhaopeng Cheng   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 08:52, 29 August 2013 by Tina Zhang (Talk | contribs) (→‎Design Notes)


lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


Design Notes

Source

The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR.

References