Reporter

Part:BBa_K1088010

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-20)
Revision as of 14:55, 22 August 2013 by Andkj11 (Talk | contribs)

E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

This is the same part as BBa_K1088007, with the exception of LacI under the control of a constitutive promoter to ensure regulation of the Lac Promoter This part consists of 9 subparts:

A constitutive promoter: BBa_J23106 A strong RBS: BBa_B0030 The LacI repressor protein, regulating the lac promoter: BBa_C0012 An artificial terminator: BBa_B1002 and the rest is similar to BBa_K1088007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3937
    Illegal SapI.rc site found at 2127


[edit]
Categories
Parameters
None