Coding
HRT2

Part:BBa_K1088003

Designed by: Andreas Kjær, Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-07-10)
Revision as of 13:56, 22 August 2013 by Andkj11 (Talk | contribs)

Cis-1,4-prenyltransferase obtained from Hevea brasiliensis cDNA

This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber. The sequence have been optimized for E. coli codons and is made from the cDNA sequence "HRT2" produced by Kasem Asawatreratanakul et al. (1)

References

(1) Molecular cloning, expression and characterization of cDNA 

   encoding cis-prenyltransferases from Hevea brasiliensis

Kasem Asawatreratanakul, Seiji Takahashi, Yuan-Wei Zhang, Atiya Rattanapittayaporn, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul, and Tanetoshi Koyama


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None