Reporter

Part:BBa_K1041001

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 19:38, 14 August 2013 by Holusac (Talk | contribs)

Neomycin Resistance Coding Device + AntG promoter

This part contains the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816


[edit]
Categories
Parameters
None