Device

Part:BBa_K1210000

Designed by: Zak Stinson   Group: iGEM13_Lethbridge   (2013-08-02)
Revision as of 18:26, 2 August 2013 by Registry (Talk | contribs)

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LacI-CFP-PK401-YFP

Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If YFP coding region is read in the 0 frame, FRET can be observed. If the YFP coding region is translated in the -1 frame, there will be no FRET.

This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None