Part:BBa_K1159001

NanoLuc Luciferase in RFC[25]

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 10
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Parameters for BioBrick BBa_K1159001 automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 10: (underlined part encodes the protein)  ATGGCCGGC ... GCTACCGGTTAA  ORF from 1 to 525 (excluding stop-codon)
Amino acid sequence:   1 MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT 101 LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG
Amino acid composition: A (Ala) 4 (2.3%) R (Arg) 7 (4.0%) N (Asn) 8 (4.6%) D (Asp) 12 (6.9%) C (Cys) 1 (0.6%) Q (Gln) 7 (4.0%) E (Glu) 8 (4.6%) G (Gly) 22 (12.6%) H (His) 4 (2.3%) I (Ile) 18 (10.3%) L (Leu) 16 (9.1%) K (Lys) 7 (4.0%) M (Met) 3 (1.7%) F (Phe) 8 (4.6%) P (Pro) 6 (3.4%) S (Ser) 6 (3.4%) T (Thr) 11 (6.3%) W (Trp) 3 (1.7%) Y (Tyr) 6 (3.4%) V (Val) 18 (10.3%)
Amino acid counting:   Total number: 175 Positively charged (Arg+Lys): 14 (8.0%) Negatively charged (Asp+Glu): 20 (11.4%)	Biochemical parameters: Molecular mass [Da]: 19381.3 Theoretical pI: 5.10 Extinction coefficient at 280 nm [M-1 cm-1]: 25440 / 25503 (all Cys red/ox)
Organism specific parameters Organism E. coli B. subtilis S. cervisiae A. thaliana Mammals Estimated half-life [h] >10 Platzhalter >20 Platzhalter 30 Codon usage (CAI) good (0.66) B. subtilis good (0.62) A. thaliana excellent (0.82)
Organism specific parameters Organism: E. coli B. subtilis S. cervisiae A. thaliana Mammals Estimated half-life [h] >10 Platzhalter >20 Platzhalter 30 Codon usage (CAI) good (0.66) B. subtilis good (0.62) A. thaliana excellent (0.82)
Sequence features:
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