Coding

Part:BBa_K1159003

Designed by: TU Munich 2013   Group: iGEM13_TU-Munich   (2013-05-17)
Revision as of 11:21, 28 July 2013 by ChristopherW (Talk | contribs)

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

BioBrick: BBa_Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0
RFC standard:RFC 25 BioBrick, so ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.ORF from -8 to 1260 (excluding stop-codon)
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 ATGGCCGGCAGGTTCGAA ... GTTTATGAAACCGGT
Amino acid sequence:
  1 MAGRFEEWVKDKHIPFKLNHPDDNYDDFKPLRKIIGDTRVVALGENSHFIKEFFLLRHTLLRFFIEDLGFTTFAFEFGFAEGQIINNWIHGQGTDDEIGR
101 FLKHFYYPEELKTTFLWLREYNKAAKEKITFLGIDIPRNGGSYLPNMEIVHDFFRTADKEALHIIDDAFNIAKKIDYFSTSQAALNLHELTDSEKCRLTS
201 QLARVKVRLEAMAPIHIEKYGIDKYETILHYANGMIYLDYNIQAMSGFISGGGMQGDMGAKDKYMADSVLWHLKNPQSEQKVIVVAHNAHIQKTPILYDG
301 FLSCLPMGQRLKNAIGDDYMSLGITSYSGHTAALYPEVDTKYGFRVDNFQLQEPNEGSVEKAISGCGVTNSFVFFRNIPEDLQSIPNMIRFDSIYMKAEL
401 EKAFDGIFQIEKSSVSEVVYETG
Amino acid composition:
A (Ala)27 (6.4%)C (Cys)3 (0.7%)H (His)15 (3.5%)M (Met)12 (2.8%)T (Thr)19 (4.5%)
R (Arg)16 (3.8%)Q (Gln)14 (3.3%)I (Ile)36 (8.5%)F (Phe)31 (7.3%)W (Trp)4 (0.9%)
N (Asn)20 (4.7%)E (Glu)30 (7.1%)L (Leu)34 (8.0%)P (Pro)14 (3.3%)Y (Tyr)19 (4.5%)
D (Asp)29 (6.9%)G (Gly)31 (7.3%)K (Lys)29 (6.9%)S (Ser)22 (5.2%)V (Val)18 (4.3%)
Amino acid counting:Total number:423Positively charged (Arg + Lys):45 (10.6%)Negatively charged (Asp + Glu):59 (13.9%)
Biochemical parameters:Molecular mass [Da]:48459.2Theoretical pI:5.55Extinction coefficient at 280 nm [M-1 cm-1]:50310 / 50497
(all Cys red/ox)
Estimated half-life [h]:E. coli:>10S. cervisiae:>20Mammals:30
Codon usage (CAI):E. coli:good (0.74)S. cervisiae:good (0.75)Mammals:good (0.66)
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please read the description.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/ppatens
Parameters
None