Translational_Unit
EFE

Part:BBa_K1065000:Design

Designed by: Thomas Perli   Group: iGEM13_UNITN-Trento   (2013-06-20)
Revision as of 11:53, 24 June 2013 by Xli (Talk | contribs) (→‎Design Notes)


2-oxoglutarate oxygenase/decarboxylase (EFE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 17
    Illegal AgeI site found at 1070
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ethylene-forming enzyme that catalyses the formation of ethylene from 2-oxoglutarate. The coding sequence was taken from Pseuhmonas syringa pv. phaseolicola PK2.


GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC...

TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG

Source

xxxx

References