Composite

Part:BBa_K781003

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)
Revision as of 20:04, 6 June 2013 by Chev1n (Talk | contribs)

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[R0010][B0034] - Cohesin II-FliC Insertion Chimera

***NOTE*** There is a frame shift error. This part is not functional!

This is type II cohesin from Clostridium Thermocellum as a chimeric insertion into flagellin.

Fig. 1 The motility assay for the K781003 construct compared against controls expressing no flagellin construct and a non-chimeric flagellin construct. Inoculations made on 0.25% swimming agar.






























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2018
    Illegal XhoI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
    Illegal AgeI site found at 938
  • 1000
    COMPATIBLE WITH RFC[1000]


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