Translational_Unit
HO1

Part:BBa_K646000

Designed by: Edward Moh   Group: iGEM11_Macquarie_Australia   (2011-09-29)
Revision as of 16:18, 10 May 2013 by 127.0.0.1 (Talk) (rollbackEdits.php mass rollback)

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Hemeoxygenase with RBS

Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.


Usage and Biology

MQ New data page diagram.jpg

The BioBrick part in team Macquarie University (2011) mechanism

When coupled to a promoter BioBrick such as T7 promoter (BBa_I719005 and BBa_I712074) to produce parts BBa_K646003 and BBa_K646004, and transformed into Bl21(DE3)Tuner E. coli cells, a green colouration (require induction with ALA and IPTG) is produced.

Results - green colonies.JPG

Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green due to the production of biliverdin from the breakdown of heme - this indicates that heme oxyganse was expressed using T7 promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
Parameters
None