Part:BBa_K511826

Inducible N-Cadherin-2A-EYFP Generator (UAS-Gal4-N-Cadherin-2A-EYFP) MammoBlock Device

This MammoBlock composite device produces both the N-Cadherin cell-cell adhesion compound and the yellow fluorescent protein EYFP when induced with Gal4 transactivator variants and otherwise produces both proteins at a low, basal (OFF) level. This generator is particularly well suited to the engineering of cellular patterns given the combination of adhesion and fluorescent readout.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 126
  Illegal SpeI site found at 2965
  Illegal PstI site found at 529
  Illegal PstI site found at 1630
  Illegal PstI site found at 3241
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 126
  Illegal NheI site found at 154
  Illegal SpeI site found at 2965
  Illegal PstI site found at 529
  Illegal PstI site found at 1630
  Illegal PstI site found at 3241
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 126
  Illegal BamHI site found at 1162
  Illegal XhoI site found at 83
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 126
  Illegal SpeI site found at 2965
  Illegal PstI site found at 529
  Illegal PstI site found at 1630
  Illegal PstI site found at 3241
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 126
  Illegal SpeI site found at 2965
  Illegal PstI site found at 529
  Illegal PstI site found at 1630
  Illegal PstI site found at 3241
  Illegal NgoMIV site found at 1481
  Illegal NgoMIV site found at 3007
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1516
  Illegal BsaI.rc site found at 2073