Part:BBa_J176171

Kozak-XbaI-NLS-6His-stop

Mammalian expression vector (without a promoter) designed to add a eukaryotic RBS (Kozak) upstream (in-frame) and a nuclear localization tag, 6-histidine tag, and stop codon downstream (in-frame) of a protein coding insert. This method only works for Silver Standard (RFC 23) inserts.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 329
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  Illegal NotI site found at 292
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal BglII site found at 12
  Illegal XhoI site found at 299
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 329
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 266
  Illegal XbaI site found at 329
  Illegal SpeI site found at 249
  Illegal PstI site found at 271
  Illegal NgoMIV site found at 819
  Illegal NgoMIV site found at 2230
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1955
  Illegal BsaI.rc site found at 3755
  Illegal SapI site found at 2675