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gmR

Part:BBa_P1014:Design

Designed by: Tom Knight   Group: Part Editors   (2006-06-11)
Revision as of 17:06, 19 June 2006 by Tk (Talk | contribs) (→‎Design Notes)


gentamicin resistance cassette aph(2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR primers
    • gtttcttcgaattcgcggccgcttctagag gagataggttatgcaagat,P1014-F
    • gtttcttcctgcagcggccgctactagta gattccggattctaaaaaaagg,P1014-R2 (after terminator)
    • gtttcttcctgcagcggccgctactagta ctatttaattttaatgcttctg,P1014-R3 (after stop codon)


  • XbaI site at 660 removed by mutating c->t which also improves codon usage.
    • gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
    • gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

DQ208936

References