Plasmid_Backbone
pSB1T3

Part:pSB1T3:Design

Designed by: Austin Che   Group: iGEM07_Example   (2008-09-08)
Revision as of 18:53, 5 February 2013 by Bcanton (Talk | contribs) (→‎Design Notes)

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High copy BioBrick assembly plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2440
    Illegal NheI site found at 1268
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2440
    Illegal BamHI site found at 1414
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2440
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2440
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2455
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1440
    Illegal NgoMIV site found at 1808
    Illegal NgoMIV site found at 1968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The ampicillin resistance marker needed to be eliminated.

Based on conversation with Austin Che, I believe the Tet marker in this plasmid was cloned in a non-directional fashion between two XhoI sites. As a result, the Tet marker in the Registry version of this plasmid could be in either orientation. Sequencing of the Registry clone would confirm which orientation is present.

Source

Austin Che constructed this plamid from pSB1AT3-P1010.

References