Part:BBa_M36071

K12 e.coli native Manganese Superoxide Dismutase

Coding sequence for the enzyme Mn-SOD. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.

Usage and Biology

Superoxide dismutase cataylyzes the dismutation of superoxide. Superoxide is produced naturally in the respiration process. It causes damage to the cell by altering lipid structure and breaking down proteins among other negative effects.

Design Notes

This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.

Source

This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 346
  Illegal PstI site found at 283
  Illegal PstI site found at 593
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 346
  Illegal PstI site found at 283
  Illegal PstI site found at 593
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 346
  Illegal XhoI site found at 43
  Illegal XhoI site found at 127
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 346
  Illegal PstI site found at 283
  Illegal PstI site found at 593
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 346
  Illegal PstI site found at 283
  Illegal PstI site found at 593
  Illegal NgoMIV site found at 542
  
• 1000
  COMPATIBLE WITH RFC[1000]