Composite

Part:BBa_M36344

Designed by: Chris Aholt, Arjun Bhargava, Nikhil Bhargava   Group: Stanford BIOE44 - S11   (2012-12-04)
Revision as of 01:32, 5 December 2012 by Nb (Talk | contribs)

Actuator for Grape Scent

This part is an actuator for the aroma normally associated with grape soda and consists of a RBS, CDS, and transcriptional terminator. The RBS uses a bicistronic design architecture, which facilitates linkage to promoter regions and allows for similar levels of protein expression across multiple environments. Our coding DNA sequence produces the AMAT enzyme (Anthraniloyl-coenzymeA: methanol acyltransferase), which catalyzes the reaction between anthraniloyl coA and methanol to produce methyl anthranilate, which is an aromatic compound responsible for the grape smell. The transcriptional terminator was specifically chosen as it is complementary to the RBS used.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1274
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1274
    Illegal NgoMIV site found at 1048
    Illegal AgeI site found at 578
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None