Part:BBa_K747102:Design

pTAL-AID

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 3200
  Illegal XbaI site found at 3253
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1999
  Illegal SpeI site found at 3201
  Illegal PstI site found at 3215
  Illegal NotI site found at 3208
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3241
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 3201
  Illegal XbaI site found at 3253
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3253
  Illegal SpeI site found at 3201
  Illegal PstI site found at 3215
  Illegal NgoMIV site found at 3823
  Illegal NgoMIV site found at 5106
  Illegal NgoMIV site found at 5389
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2071
  Illegal BsaI.rc site found at 1417
  Illegal BsaI.rc site found at 2233
  Illegal BsaI.rc site found at 2722
  Illegal BsaI.rc site found at 5601
  Illegal SapI site found at 2823
  Illegal SapI.rc site found at 4955
  Illegal SapI.rc site found at 5165
  

Design Notes

see main page

Source

see for further information: Eukaryotic TAL expression plasmid

The TAL-protein was build with the following parts:

BBa_K747009: GC

BBa_K747026:GG

BBa_K747045:TC

BBa_K747060:TA

BBa_K747079:TT

BBa_K747081:AC

The AID was constructed by the IGEM 2012 team of Potsdam: modified AID

References

1. Scholze, H. & Boch, J. TAL effectors are remote controls for gene activation. Current Opinion in Microbiology 14, 47–53 (2011).

2. Moscou, M. J. & Bogdanove, A. J. A Simple Cipher Governs DNA Recognition by TAL Effectors. Science 326, 1501–1501 (2009).

3. Cermak, T. et al. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 39, e82 (2011).

4. Reyon, D. et al. FLASH assembly of TALENs for high-throughput genome editing. Nature Biotechnology 30, 460–465 (2012).

5. Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nature biotechnology 29, 149–153 (2011).

6. Miller, J. C. et al. A TALE nuclease architecture for efficient genome editing. Nature Biotechnology 29, 143–148 (2010).

7. Boch, J. et al. Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors. Science 326, 1509–1512 (2009).

8. Liu, J. et al. Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy. Journal of Genetics and Genomics 39, 209–215 (2012).

9. Wood, A. J. et al. Targeted Genome Editing Across Species Using ZFNs and TALENs. Science 333, 307–307 (2011).

10. Sander, J. D. et al. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat Biotechnol 29, 697–698 (2011).

11. Tesson, L. et al. Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology 29, 695–696 (2011).

12. Hockemeyer, D. et al. Genetic engineering of human pluripotent cells using TALE nucleases. Nature Biotechnology 29, 731–734 (2011) 13. Sanjana, N. E. et al. A transcription activator-like effector toolbox for genome engineering. Nature Protocols 7, 171–192 (2012).