Translational_Unit

Part:BBa_K917009:Experience

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-08-10)
Revision as of 11:42, 30 October 2012 by Krackus (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K917009

We have tested the expression of CymA protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and cymA gene). Cell pellets were scanned prior to sonication and cymA transformed cells show slightly more intense colour, indicating higher concentration of haem in cells. Cytochrome compare.jpg

Haem staining results suggest CymA expression in E coli transformed with plac-cymA and plac-ccm. The additional band indicated by an arrow in lane 3 represents CymA protein. Gel haem compare .jpg

User Reviews

UNIQe194f8b8df982a75-partinfo-00000000-QINU UNIQe194f8b8df982a75-partinfo-00000001-QINU