Translational_Unit

Part:BBa_K917003:Experience

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-08-10)
Revision as of 11:41, 30 October 2012 by Krackus (Talk | contribs)

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Applications of BBa_K917003

We have tested the expression of NapC protein under control of lac promoter in ccm transformed cells (ccm gene cluster BioBrick was transferred into pSB4K5 vector to allow for double epxression of ccm and napC gene). Cell pellets were scanned prior to sonication and napC transformed cells show slightly more intense colour, indicating higher concentration of haem in cells. Cytochrome compare.jpg

Haem staining results suggest NapC expression in E coli transformed with plac-napC and plac-ccm. The additional band indicated by an arrow in lane 7 represents NapC protein. Gel haem compare .jpg

User Reviews

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