Coding

Part:BBa_K775000

Designed by: Sietske Grijseels   Group: iGEM12_TU-Delft   (2012-08-31)
Revision as of 20:50, 29 October 2012 by MarkWeijers (Talk | contribs)

RI7-GPR109A Niacin chimeric GPCR

BBa_K775000 encodes a chimeric niacin-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mammalian GPR109A, flanked by the N- and C- terminals of the rat OR RI7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 478



Immunolocalization of the receptor protein using FLAG treatment. A halo formation indicates appropriate localization in the membrane




Flow cytometry results of cells stained with Vybrant® DyeCycle™ Orange DNA Stain after 4.40 hours after activation with a saturative amount of nicotinic acid. In the middle and right picture a shift of peaks can be observed, corresponding with formation of more haploid cells (left) in comparison with diploid cells (right).

[edit]
Categories
//chassis/eukaryote/yeast
Parameters
n/a RI7-GPR109A Niacin chimeric GPCR