Part:BBa_K902084

Prha-B0034-S7(active)

This part the Prha promoter, which is repressed by glucose and induced by rhamnose upstream of S7 micrococcal nuclease. This is an active killswitch where upon induction with rhamnose, the nuclease is produced, resulting in cell death. Conversely, when glucose is added, the promoter is repressed, causing no expression of the nuclease gene, resulting in the cells being able to grow. We characterized this circuit using differing concentration of glucose and rhamnose. The results show that with increasing concentrations of glucose, this killswitch is deactivated. The Prha promoter was initially characterized via fluorescense using GFP. This data can be found here. Please see our Wiki for more information!

Figure 1. pRHA-S7 construct demonstrating our kill switch in TOP10 wild type cells and glyA knockout cells. This demonstrates that our system is capable of being induced by the sugar rhamnose and repressed in the presence of glucose. There is no growth in rhamnose with our system as the RhaBAD operon has been deleted in the knockout strain we are using..

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]