Composite

Part:BBa_K902084

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-25)
Revision as of 06:42, 28 October 2012 by Emily Hicks (Talk | contribs)

Prha-B0034-S7(active)

This part the Prha promoter, which is repressed by glucose and induced by rhamnose upstream of S7 micrococcal nuclease. This is an active killswitch where upon induction with rhamnose, the nuclease is produced, resulting in cell death. Conversely, when glucose is added, the promoter is repressed, causing no expression of the nuclease gene, resulting in the cells being able to grow. We characterized this circuit using differing concentration of glucose and rhamnose. The results show that with increasing concentrations of glucose, this killswitch is deactivated. The Prha promoter was initially characterized via fluorescense using GFP. This data can be found here. Please see our Wiki for more information!

Calgary Rha S7 Data.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None