Part:BBa_K792004

Import enhancer - Polyarginine (trojan peptide)

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process. They can be used to increase the efficiency with which a protein enters a cell.

This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.

This part includes a HindIII restriction site as an extra.

(Refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)

See also:

Another Trojan Peptide part BBa_K792003

Trojan peptides can penetrate cell membranes. Extreme your safety measures when dealing with strains expressing this part: avoid direct contact with them or conditioned medium and discard them as pathogenic residues.

This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]