Device

Part:BBa_K812012

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-18)
Revision as of 00:09, 27 October 2012 by Landrain (Talk | contribs)

OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.

This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.

This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.

This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
    Illegal NotI site found at 287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1648
    Illegal XhoI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


[edit]
Categories
Parameters
None