![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K902080:Design
Designed by: Lisa Oberding, Ali Honarmand Group: iGEM12_Calgary (2012-10-25)
PlacI/dszB/B0034/dszC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1428
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 652
Illegal BamHI site found at 2232
Illegal XhoI site found at 644 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 359
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2382
Design Notes
DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info
dszB and dszC were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info
Source
Assembled from biobrick parts
References
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21