Generator

Part:BBa_K902080:Design

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-25)
Revision as of 23:42, 26 October 2012 by Lisa.O (Talk | contribs)

PlacI/dszB/B0034/dszC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 652
    Illegal BamHI site found at 2232
    Illegal XhoI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 359
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2382


Design Notes

DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info

dszB and dszC were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info


Source

Assembled from biobrick parts

References

Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21