Generator
Part:BBa_K902041
Designed by: Jeff Addison Group: iGEM12_Calgary (2012-10-24)
Revision as of 21:19, 26 October 2012 by Jeffaddison89 (Talk | contribs)
Constitutive AmdA Generator
AmdA coding sequence (K902035) downstream of a strong TetR repressible promoter (R0040) and a ribosome binding site (B0034). AmdA is an amidase enzyme from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol.
It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid. This part's function was characterized using benzamide as a model compound as detailed in the experience section of this page.
Sequence and Features
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Categories
Parameters
| None |